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KMID : 0383820080640040278
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Tuberculosis and Respiratory Diseases
2008 Volume.64 No. 4 p.278 ~ p.284
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Immunohistochemical Analysis for the Expression of DR5 TRAIL Receptor and p53 in Non-small Cell Lung Cancer
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Lee Kye-Young
Lee Jung-Hyun
Kim Sun-Jong
Yoo Kwang-Ha
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Abstract
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Background: TRAIL is a promising anticancer agent which induces selective tumor cell death due to a unique
receptor system that includes death receptors and decoy receptors. DR5 TRAIL receptor is an originally identified
p53-regulated death receptor gene that was induced, by doxorubicine, only in cells with a wild-type p53 status.
We investigated that focused on the correlation between the DR5 and p53 expressions in non-small cell lung cancer
(NSCLC).
Methods: Immunohistochemical analysis, with using avidin-biotinylated horseradish peroxidase complex, was
carried out in 89 surgically resected NSCLC formalin-fixed paraffin-embedded tissue sections. As primary antibodies,
we used anti-DR5 polyclonal antibody and anti-p53 monoclonal antibody. A negative control was processed with
each slide. The positive tumor cells were quantified twice and these values were expressed as percentage of the
total number of tumor cells, and the intensity of immunostaining was expressed. The analysis of the DR5 expression
was done separately in tumor area and in a nearby region of normal tissue.
Results: The DR5 expression was high in the bronchial epithelium (89% of cases) but this was almost absent in
type I & II pneumocytes, lymphocytes and smooth muscle cells. High DR5 expression rate in tumor was seen
in 28% (15/53) of squamous cell carcinomas, in 47% (15/32) of adenocarcinomas and, in 50% (2/4) of large cell
carcinomas. The DR5 expression did not show any statistical significance relationship with the T stage, N stage,
or survival. However, the DR5 expression showed significant inverse correlation with the p53 expression. (p＜
0.01).
Conclusion: We demonstrated that the DR5 expression in NSCLC via immunohistochemical analysis is relatively
tumor-specific except for that in the normal bronchial epithelium and it is significantly dependent on the p53
status. This might be in vivo evidence for the significance of the DR5 gene as a p53 downstream gene. (Tuberc
Respir Dis 2008;64:278-284)
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KEYWORD
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TRAIL, DR5, p53, NSCLC
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